yoaB [2019-07-02 11:01:24]

2-oxoglutarate permease (proton symporter)

Locus: BSU18540

Isoelectric point: 9.41

Molecular weight: 45.22 kDa

Protein length: 414 aa

Gene length: 1245 bp

Function: uptake of alpha-ketoglutarate

Product: 2-oxoglutarate permease (proton symporter)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Transporter for organic acids
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,021,223 → 2,022,467

The protein

Protein family

• major facilitator superfamily (according to UniProt)
• Sugar transporter (TC 2.A.1.1) family (according to UniProt)
• CsbX Subfamily (with CsbX, according to UniProt)

Paralogous protein(s)

• CsbX

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: yoaD-yoaC-yoaB
  Description: PubMed

  Regulatory mechanism

  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• MGNA-A833 (yoaB::erm), available at the NBRP B. subtilis, Japan
• BKE18540 (ΔyoaB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCTGTCTCCTCCTTAG, downstream forward: _UP4_TAATTAGAAAGCGCCCTGTT
• BKK18540 (ΔyoaB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCTGTCTCCTCCTTAG, downstream forward: _UP4_TAATTAGAAAGCGCCCTGTT

References

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